LOCUS BG085604 712 bp mRNA linear EST 12-MAY-2010 DEFINITION H3115C12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3115C12 5', mRNA sequence. ACCESSION BG085604 VERSION BG085604.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 712) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085604.1. Other_ESTs: H3115C12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3115 row: C column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..712 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3115C12-5" /db_xref="taxon:10090" /clone="H3115C12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 155 a 226 c 178 g 153 t ORIGIN 1 ggcaactgaa gctggatgag gagcctcctg gccaaagcag tcggtgttgc ccagccctgg 61 agacacgagg ccgtgggacc cctcagctag ctgaggaagc tacagccaaa gccatcagcg 121 acctagctgt gcgtagggcc cgtcaccggc tgctctctgg ggactctata gagaagcgca 181 ccactcgccc tgtcaacaaa gtaatcaagt cagcctcagc tacggccctt tccctcctca 241 ttccttcaga acaccatgcc tgctcaccat tggccagccc tatgtcccca cattcccagt 301 catccaatcc atcatccagg gactcttctc caagcaggga cttcttgcca gcccttggca 361 gcttgaggcc tcccatcatc atccaccgag ctggcaagaa gtatggcttc accctgcggg 421 ccattcgagt ctacatgggt gacactgatg tctacaccgt acaccacatg gtgtgggtat 481 gtctggccag acagagttgt tgtcctccag ctgtgtactc ccaccttccc cttaccagag 541 cctatctgcc ttttctttgg tcactgccca cataactcac agaaaccaga gggtggtggt 601 agtcaagggt cacaacagca gcagaaccag gctcccgatt aaaggctttt ctcttcctga 661 ggcagcatgt ggaggatggt ggtccagcca gtgaagcagg gcttcgtcag gg //