LOCUS BG085206 649 bp mRNA linear EST 12-MAY-2010
DEFINITION H3109G10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3109G10 5', mRNA sequence.
ACCESSION BG085206
VERSION BG085206.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 649)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG085206.1.
Other_ESTs: H3109G10-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3109 row: G column: 10
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..649
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3109G10-5"
/db_xref="taxon:10090"
/clone="H3109G10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 138 a 179 c 191 g 141 t
ORIGIN
1 gcggcggggc cgggcgctcc tcgccttggt agcttcgctg ctcctctccg gggcccaggt
61 ggccagccga gaactagacg tccacgagaa caccactgat gacatggcca ggaacaggaa
121 tggagccgac tcttctgtcc tgagtgttcc cagaaagcag agtgctgagg acctgtctgc
181 tgagatcttc aactatgaag aatactgtgt cccgaaggcc gtcactgggc cgtgccgcgc
241 agccttccct cgctggtact atgacactga aaagaattcc tgtatcagct tcatctacgg
301 cgggtgcgga ggcaacaaga acagttacct ctcccaggaa gcatgcatgc agcactgctc
361 tggtaagcag atgcatcctt tcctgacccc gggcctaaaa gcggtgatcc tggtggggct
421 gttcctcatg gttctgatcc tcctcctggg aacctctatg gtctgcctca tccgtgtggt
481 tcgcagaaag caggagcgtg cgctgcgcac tgtttggagc actgcggatg acaaggagca
541 gctggtgaag aacacctgtg tcttgtagag gccccagtgc tcagaaccgg gagtgacaac
601 ttgtgctgtt taaaaagaga gcaactgctt gcgtctgaga tcattaggg
//