LOCUS BG085193 652 bp mRNA linear EST 12-MAY-2010 DEFINITION H3109F05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3109F05 5', mRNA sequence. ACCESSION BG085193 VERSION BG085193.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 652) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085193.1. Other_ESTs: H3109F05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3109 row: F column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..652 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3109F05-5" /db_xref="taxon:10090" /clone="H3109F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 146 a 161 c 153 g 192 t ORIGIN 1 tagctaccca ctcacccatc catccatcca tccatccatc catccatcca tctttccatc 61 cactcatcca ggaacagaaa attggggagt tcttctggag ttcttaattt tgtctgggga 121 tacagatgaa aagagaacat ctctccctta actgttggct ttggctttgg gcagtgagcc 181 tcagatgaga atgttgtatg gcttgtgtta ccctatgtgc agtttactgc cttactgtct 241 tttatggctc ccagagccgt tgctgctttt gtagatgtaa tatttggttc tttctactta 301 gcctgactgt gggaactagg catgagaacc tgctgtccca gctagcaata atccctgtgc 361 gtgcacagta ggctatagct aggaagggtc ttcaccccaa ttatctcagc tgattctctc 421 ccctagtctt ggatgaaggg tttactgtgg ggcaggtgac caagaccatg aacttggtca 481 ctgaactgtg gaacagggag aaacgtggag cttaggctat gagcacagga catttgcctc 541 gtaatccaag tgcccctgtg ttttgtgtct ctgctgagca cgagcacagt gtcctctgaa 601 tgcaaatacc ggtttgagtg caaggctgct atgttctccc ctgcatggac ac //