LOCUS BG085135 661 bp mRNA linear EST 12-MAY-2010
DEFINITION H3109A06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3109A06 5', mRNA sequence.
ACCESSION BG085135
VERSION BG085135.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 661)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG085135.1.
Other_ESTs: H3109A06-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3109 row: A column: 06
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..661
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3109A06-5"
/db_xref="taxon:10090"
/clone="H3109A06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 160 a 147 c 157 g 197 t
ORIGIN
1 cacctgtggg agtcctgtct ctctcttcat catgtgggct cttggaatct aactcctgtt
61 gcctggcttg gtgacagtgc tttctcagca gctgttcgag ttagtgttag tgtgtctgtg
121 agggactgtc ttgactgaaa gctgatgtag aagggcccag ctgattgtat gtggcaccat
181 ccctaggcag gtggtcctgg actgtagaag gaagcatggc ccctgaactt tagcctacag
241 aagccagagg gtgagccagc gagtgggatt cctccataat gtctgcttca ggttcctact
301 ttgagttcct gtcataactt cccacagcta cagattgtga tcttaaagta taaaccaaat
361 gaatcctttc ctcactgaag ttgcttttgg ctaaagagtt tttaatctca gcaatggaag
421 actggactgt tagccagaca tctttggatg aacttgctcc ctacctcttt aattttcatg
481 attagattct tttctcgtat tgatcttaga gaactttaaa aacgtattct gaagacaagt
541 gtggtagtac aaacttatga gccaggtact caggaagccc agagagacct tgggttcaag
601 gccaacctgc actacatagt gagttcaagg ccagcctggt gaacatatca aggcctttct
661 c
//