LOCUS BG085040 409 bp mRNA linear EST 18-DEC-2003 DEFINITION H3107H12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3107H12 5', mRNA sequence. ACCESSION BG085040 VERSION BG085040.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 409) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT On Jan 26, 2001 this sequence version replaced BG085040.1. Other_ESTs: H3107H12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3107 row: H column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..409 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3107H12-5" /db_xref="taxon:10090" /clone="H3107H12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 112 a 102 c 101 g 94 t ORIGIN 1 ggaaaagttc tccccgctga cggccaacct catgaattta cttgctgaaa atggtcgcct 61 aggcaacacc cagggtatca tctctgcctt ttccaccatc atgagtgtcc accgcggaga 121 agtgccatgc acagtgacca cagcatctcc tctagatgac gctgttctct ctgagttaaa 181 gacggtgctg aagagcttcc tgagtccaaa ccaaatactg aaactggaga tcaagactga 241 cccgtcaatc atgggcggga tgattgtccg aattggggaa aagtacgttg atatgtctgc 301 aaagagcaag attcagaagc tcagcaaggc catgcgggag atgctctgag agactgtcac 361 ctgtgtgagc tcttgtcctt ggagcaacaa taaaatgctt cctgaacag //