LOCUS BG084950 622 bp mRNA linear EST 12-MAY-2010
DEFINITION H3106H06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3106H06 5', mRNA sequence.
ACCESSION BG084950
VERSION BG084950.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 622)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG084950.1.
Other_ESTs: H3106H06-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3106 row: H column: 06
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..622
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3106H06-5"
/db_xref="taxon:10090"
/clone="H3106H06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 157 a 144 c 138 g 183 t
ORIGIN
1 ctcaaagttg accaattcta tttatagtac tgtttgaatt attttccact agatcacagt
61 aactgttctt ggaagactta ggtctccaga actcctacta ggtttggaag tagctgctgt
121 ttgcggctac tgtcatccac tgaatcattt accaattgct tgggtgggtc agaggtctct
181 tgtcagatca tggtcctgca caggataatt ttgaggacaa cagatcctgc ctgaggtcac
241 atgccctctt gacagcaaat gctccttgtt ctgccgtgtc ccctctcttt tccccagctc
301 aagattgtgg ccttactcag atgccctgtg catgtgaatc cctactgtaa ttgctactgg
361 actcctttgc cagagtgcta gcagccaggg ctgctggagc tgcagtctga tgagaagcct
421 tgttttcctc tgagcactgc ttaaaaatag tgactaggct tcttattact gtacgaacag
481 aattgtacta aaaaggggac tttttatagt ttaaatgtgg aagccctgac tacataaagc
541 ctactctgtt cctctcacag aggacccact cagtccgaat gaaggaaaac caatgaggga
601 actagggaat agtagcaaag gg
//