LOCUS BG084370 589 bp mRNA linear EST 12-MAY-2010 DEFINITION H3100A07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3100A07 5', mRNA sequence. ACCESSION BG084370 VERSION BG084370.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 589) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG084370.1. Other_ESTs: H3100A07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3100 row: A column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..589 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3100A07-5" /db_xref="taxon:10090" /clone="H3100A07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 154 a 125 c 137 g 173 t ORIGIN 1 ctaagccatg cctcggcttg gataggtgca cagacaaact agccctgacc gcatttacgt 61 ccagcatctc tttagaaacc tcaccttgga atgtagctat ggtcagagac tctgagtgtt 121 acaacagagg gcacaatggg cagtgtgcct tttttttttt ttaagatgga aaagctgcat 181 aggaaacatc tgattagaaa caaatagctc cagttgctat aggttctata taagtgttct 241 actcatagcc acactccccc tagcctctag accttcatac attaccagag gtgacctgcc 301 aaaagcagaa aagcctttgt catggtgcct ttttatttgt ttatgattta tttattccat 361 ttctttattt tgtatgtatg gggtagggga tatgtgtgac acagtgagcc tatgggggcc 421 agagggaaac ttccagagtt tgttttctgg aactaggtag catgagagct ctggggatct 481 taggttaaga ctggcagcaa acaccctgac cagctgactg ttttacactg agtgttggag 541 tcaggcatga tgacatactc ctactatcct agcgcctggc aggtaagac //