LOCUS BG084284 562 bp mRNA linear EST 12-MAY-2010
DEFINITION H3098F09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3098F09 5', mRNA sequence.
ACCESSION BG084284
VERSION BG084284.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 562)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG084284.1.
Other_ESTs: H3098F09-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3098 row: F column: 09
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..562
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3098F09-5"
/db_xref="taxon:10090"
/clone="H3098F09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 135 a 138 c 169 g 120 t
ORIGIN
1 caggagccgg agtggctgga gtcgcgggat ggtcaggagg cgggctgaaa gttggagtaa
61 gcaggaagtg aagctgtggc tgccttgggt ggaaacagac taggcgaagt tctgctttga
121 gaggcccaag tgctccgaga catggaggag gccagtggga gtgccggaaa tgaccgagtt
181 aggaacctgc agagtgaggt ggagggagtc aagaatatta tgacccagaa tgtggagcgg
241 atcttggcca gaggggagaa cctggaccac ctccgaaaca agacagagga cttggaagcc
301 acgtctgaac acttcaagac aacgtcccag aaggtggccc ggaagttctg gtggaagaat
361 gtgaagatga ttgtcatcat ctgtgtgatt gtccttatca tcgtcatcct cattatactt
421 tttgccactg gtaccatccc cacttaagag tcccttccat cccaccctac tcctcagggg
481 tgacctccct aatatgtgcc aagaggagcc ctctcctgtc ctcctccacc caagtgctgc
541 tcagtccctg tgagggcctt ct
//