LOCUS BG084177 674 bp mRNA linear EST 12-MAY-2010 DEFINITION H3097C10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3097C10 5', mRNA sequence. ACCESSION BG084177 VERSION BG084177.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 674) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG084177.1. Other_ESTs: H3097C10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3097 row: C column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..674 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3097C10-5" /db_xref="taxon:10090" /clone="H3097C10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 235 a 143 c 146 g 150 t ORIGIN 1 ctcaagactt gtctgtatct ctgcagtgaa gcctttagaa caaactctac aaccatggag 61 gagacttcaa gaaactcagt ttcaagccag cctttgatag gaagtctcac aagaagtatg 121 atactcaaca aattacagaa gagaagagag acccagattg tcccgaaaaa gataaaggcc 181 aaacatagaa ctggtgttac ttccaaagat tctatgagga tgaccagttg catctttgca 241 aagccagtga ccacaataac ttcataccct gagaatgaaa ccaggtacag gagagaggaa 301 gataagctga caaaacccaa gcagctctgt gcacttaaga gactgaagaa acatcaagtt 361 ggagagaata aacaggacct gtcatgtcaa ttgaagctta caaaccctgt agaaagaatt 421 gcactgggaa tgcgggatga aactaatgac caaagtggtg ttaagggtca gcttactcct 481 ggagaagtta cctctgtcca aaccccatgc ttggaaaaaa atgaacaaat ggcttttcag 541 ctgtcaccat ttttctccag tccaggggta acaatgccag tgcgtcttca tctgtcacca 601 tcttacttca tccaagaagt aactattgca gacatcctga gtcagatctg ggaggtaaag 661 aaagccagga agag //