LOCUS BG084174 506 bp mRNA linear EST 12-MAY-2010 DEFINITION H3097C07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3097C07 5', mRNA sequence. ACCESSION BG084174 VERSION BG084174.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 506) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG084174.1. Other_ESTs: H3097C07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3097 row: C column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..506 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3097C07-5" /db_xref="taxon:10090" /clone="H3097C07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 101 a 106 c 107 g 192 t ORIGIN 1 ctcctgtgct gcttccaagt gtgagtgaga ccaatggttg tgattggtaa gtggctcacg 61 ggatgttttt atttgtagac attacctcca tgtcatgtct ctgtacagca cactgcagtg 121 gtgtgcaagc tgatgcaggg gtttctgcct tccctgtgtg aagtggcttg atttccctcc 181 atctggatgc taatgctgtg tcccatttcc attaatgagc tactgagtaa atcagctcaa 241 accctgccag attgtctctg gcagcagctc ctaataatta tttatgcttt gggaatcttt 301 ttgtcggctt ctagaacctt ccgtccactc tcagcttagg tttttgattt gtgtaatata 361 tgtatattct ttttccttgc tgtcgtcctc cctgcctctc cgttttgttt cttaaaatgt 421 tctgtagttt tgtacaagat gagacttagc cttgggtact tcttgctaaa gctttaatgc 481 tttgtaaata aaattggatg tttatt //