LOCUS BG083858 706 bp mRNA linear EST 12-MAY-2010 DEFINITION H3092H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3092H10 5', mRNA sequence. ACCESSION BG083858 VERSION BG083858.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 706) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083858.1. Other_ESTs: H3092H10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3092 row: H column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..706 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3092H10-5" /db_xref="taxon:10090" /clone="H3092H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 194 a 180 c 154 g 178 t ORIGIN 1 gtcacttact cagtattcca aatgtgtgtt catggatgca gatactctgg tcctctcaaa 61 tattgatgat ctgtttgaaa gagaagaatt atcagcagca ccagacccag ggtggcctga 121 ctgtttcaat tctggagttt ttgtctatca accctccatt gaaacgtata accagctgtt 181 gcatctggct tctgagcaag gtagttttga tggtggggac cagggcctac tgaatacata 241 ttttagtggc tgggcaacga cagatatcac aaaacacctg ccatttgtgt ataacctaag 301 cagcatttca atatacccct acctcccggc atttaaagcg tttggtaaaa atgccaaagt 361 tgtgcacttc ctgggacgaa ccaagccatg gaattacact tataaccccc aaacaaaaag 421 tgtcaactgt gactcccagg acccaaccgt gcgtcaccca gagtttctga acctgtggtg 481 ggacaccttc accaccaacg tcttacccct gctccaacac catggccttg tcaaagatgc 541 cagctcctat ctaatgatgg aacatgtctc aggagccttg tcagacctgt cctttgggga 601 ggccccagct gcaccacaac cttctatgtc ctcagaagaa aggaaggaac ggtgggaaca 661 gggccaggcc gattacatgg gagcagattc ttttgataac atcaag //