LOCUS BG083594 579 bp mRNA linear EST 12-MAY-2010 DEFINITION H3089A12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3089A12 5', mRNA sequence. ACCESSION BG083594 VERSION BG083594.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 579) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083594.1. Other_ESTs: H3089A12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3089 row: A column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..579 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3089A12-5" /db_xref="taxon:10090" /clone="H3089A12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 139 a 153 c 150 g 137 t ORIGIN 1 ctggtggcta ctttcatttt tcccctctcc tctctgaata cttacagctc ccataagtag 61 gcacacatgg gccttgtact caggaaaatg ctgtccagtg gggtgtgcac atctaatgtt 121 cagcttcccg ggaaggtagc cacagtcact ggtgctaacg caggcattgg gaaggagaca 181 gctaaagatc tggcccaaag aggagcccgt gtgtatttag cttgccggga tgtggacaag 241 ggggaactgg cggctcgtga gatccaagcc gtcacaggga acagtcaggt cttcgtacgg 301 aaactggacc tagctgatac caagtctatt cgagcctttg ccaaagactt cttagctgag 361 gaaaagcatc cgcaccttct catcaacaat gcgggcgtga tgatgtgccc ctactcgaag 421 actgcagacg gctttgagat gcacattggc gtcaaccacc tgggtcactt cctcctgacc 481 catttgctgc tagaaaagct gaaggagtcg gccccatcaa ggatagtcaa cttgtcttct 541 ttgggacacc atctgggcag gatccacttc cataacctg //