LOCUS BG083400 698 bp mRNA linear EST 12-MAY-2010
DEFINITION H3087G12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3087G12 5', mRNA sequence.
ACCESSION BG083400
VERSION BG083400.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 698)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG083400.1.
Other_ESTs: H3087G12-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3087 row: G column: 12
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..698
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3087G12-5"
/db_xref="taxon:10090"
/clone="H3087G12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 216 a 118 c 170 g 194 t
ORIGIN
1 ctggggcaga tagactcttc agatgaggag gaccaggatg ggagtcaagg cttgggcaag
61 agaaagaggg tgaagctaag cagtggcacc aaagatcaat ccataatgga tgttttgaaa
121 cataaaagtt ttttagaaga actattattt tggacaataa agtatgagtt cccacagaag
181 atggtaactt tcttgctcaa tatgctccca gatcaagagt ataaggttgc ttttacaaaa
241 acatttgttc agcattatgc ttttattatg aaaacactga agaaaagtca cgaatcagat
301 acaatgtcca acagaattgt gcatattagt gttcagttgt tcagcaatga ggagctagcc
361 agacaggtga cagaagagtg tcagctcctg gacatcatgg tcactgtgtt gttgtacatg
421 atggagagct gcctcatcaa gagtgagctg caagatgaag agaacagttt acatgtagtg
481 gtgaactgtg gagaagcctt actgaagaat aacacctact ggccccttgt cagtgatttt
541 attaatattc tttctcatca aagtgtggct cagaggtttc tggaggatca cggcctgtta
601 gttacatgga tgaactttgt gtctttcttt caaggtatga acttgaacaa gcgagagctc
661 aatgagcacg tggaatttga gtctcagacc tactatgc
//