LOCUS BG083365 701 bp mRNA linear EST 12-MAY-2010 DEFINITION H3087D12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3087D12 5', mRNA sequence. ACCESSION BG083365 VERSION BG083365.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 701) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083365.1. Other_ESTs: H3087D12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3087 row: D column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..701 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3087D12-5" /db_xref="taxon:10090" /clone="H3087D12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 144 a 220 c 205 g 132 t ORIGIN 1 tccgctcagc cctgcgccac ctcccctcag agtggctcct gggagagtga tgcagagtcc 61 ctgtcgccct gcccacccca gcgccgcttc tccctgtcac ccagcctggg cccacaggcg 121 agccgcttcc tgccctctgc ccggagctct cccgcgtctt ccccagagct gccctggaga 181 ccccgaggtc tccgaaacct tccccgaagc cgctcacagc cttgtgatct ggatgcccgc 241 aaaactggtg tcaagcggcg tcatgaggaa gactgcaggc gccttcggcc ttccttggac 301 tttgacaaga tgaatcagaa accatactca ggaggtctct gtctccaaga aacagcccga 361 gaagaaggca gcaacgtctc tccaccatgg ttcatggcct gtagtccccc acctctctct 421 gcttcctgca gtcccgtcga gggttcatcc caggtgctga gtgaaagcga agaggaggaa 481 gaggggtctg tgcggtggga acggcaggca ctgagcaagc ggacattgtg ccagcaggac 541 tttggggacc tggacctgaa tctgattgaa gaaaactaaa agtgagaggc tgcttcctgg 601 gcccatgcag actgtgtaat ggttactaac acgtgtgagg gccccaaggc tgagggccca 661 gcctgggaaa gggggtgaat ggagggctcc tactcagggc a //