LOCUS BG083340 646 bp mRNA linear EST 12-MAY-2010 DEFINITION H3087B09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3087B09 5', mRNA sequence. ACCESSION BG083340 VERSION BG083340.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 646) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083340.1. Other_ESTs: H3087B09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3087 row: B column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..646 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3087B09-5" /db_xref="taxon:10090" /clone="H3087B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 181 a 149 c 175 g 141 t ORIGIN 1 ctggcaggct cagagaggct cagcaagact gggtctgaag gtagagtcct aaaggaagcc 61 acctacatca acaagtcact gtcattcctg gagcaggcca tcattgccct tggggaccag 121 aatcgggacc acgtcccttt ccggcagagc aagctcaccc atgccctgaa agactcctta 181 gggggaaact gtaatatggt cctcgtgaca aacatctatg gagaagctgc ccagttggat 241 gaaacgctgt cttcattgag gtttgccagc aggatgaagc tggtcaccac tgagcctgcc 301 atcaatgaga agtatgatgc tgagagaatg gtcaagaatt tggagaagga actggcattg 361 cttaagcagg aactggccat ccatgatagt ctgtccaacc gcacccttgt gaattatgac 421 cccatggatg aaattcagat tgctgagatc aactctcaag tacggaggta cctggagggg 481 acgctggacg agattgacat aatcaatcta aggcagattc aggaggtatt caaccagttc 541 agggtggttc tgagccaaca ggagcgggaa gtagagtctg ccttgcgcag gaagtacact 601 ctcatagaca aaaatgattt tgcagccatt tctgctgtcc agaagg //