LOCUS BG083070 528 bp mRNA linear EST 12-MAY-2010 DEFINITION H3084B10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3084B10 5', mRNA sequence. ACCESSION BG083070 VERSION BG083070.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 528) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083070.1. Other_ESTs: H3084B10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3084 row: B column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..528 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3084B10-5" /db_xref="taxon:10090" /clone="H3084B10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 152 a 146 c 141 g 89 t ORIGIN 1 aaagagtcgg cggagcaaaa cctgcaggcc aagaacctgc ccctggatgt ggccatcgag 61 tgcctgactc tgcgagagag ccggcgagac attgatgtgg tgagggaccc tgtggaggaa 121 gagctgctga aagaggtgga agtcattgaa gccaccaaga aggtcctaca ggagaagatc 181 agccaggcct tccagcacct ctgtctcctg caggaaattc ggcagcaact caactctgac 241 caccgggaca aaatggagac actggagatc gacaggggct gcctctctct caacctcact 301 tccccaaaca tctctctgaa agtcaacccc acacgcatcc ccaaagactc caccacactg 361 cagcagtggg atgaatttac tcgattcaac aagaaccggg cagaggccga aatgaaagcg 421 tccatagagt tgagggaagc cattgcccta gctattgctc agaccaacaa tgaactggac 481 gctcagaggg ttgcaacaga attcaccttc aggaagcggc ttcgggaa //