LOCUS BG082983 592 bp mRNA linear EST 26-JAN-2001 DEFINITION H3083B07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3083B07 5', mRNA sequence. ACCESSION BG082983 VERSION BG082983.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 592) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3083B07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3083 row: B column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..592 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3083B07-5" /db_xref="taxon:10090" /clone="H3083B07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 187 a 90 c 162 g 153 t ORIGIN 1 tagtttttaa gaatctgttg gagatcctta aggaggaaat gaaaacacaa acaaacaatg 61 gaaggaagtt aatagaactg ttcaaaatgt gaaagtggaa atagcgtcaa taaggaaagc 121 cccgaagtgg aaacttattt tttctttgga aagttgcttg gatggtgttt tgctggggta 181 aacaggtaaa ggagtatttt cccgaagtgg acacagatga aagactaagg cagactcatg 241 aaggaaggtt ttgctgaagc agacacagga gagaggttgt tctgctaatt caagcatgtg 301 aaaggacacg tgatgaagga ttctttgcta acaacacgca tgtattattc tgctttacat 361 tgagctacag tactagttga gttgtatttg ttgggactcc atagagagaa aggcaccaaa 421 aaccttctgg tattgtactg cagtttcttg ctgctaccac agactcaggt tgattgacag 481 agcgtagtca cctgagacag acacatgtgc tgaggtaaga ctggtgctgg gacaaggcat 541 gtggaggacg cgtggtgtat ggagggtata aataggactt gacaggcagc ac //