LOCUS BG082966 636 bp mRNA linear EST 12-MAY-2010 DEFINITION H3082H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3082H10 5', mRNA sequence. ACCESSION BG082966 VERSION BG082966.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 636) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082966.1. Other_ESTs: H3082H10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3082 row: H column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..636 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3082H10-5" /db_xref="taxon:10090" /clone="H3082H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 146 a 173 c 194 g 123 t ORIGIN 1 gttgcaaccc ccggaggtga gggcgggacc tcgagcccag ggatgttggg ccaggaagga 61 tggaaaggga ccaggtaggc ctgctggggc ctggacccct gggtctgagg gaggagggct 121 ggggcctaga cctctgggtc tgaggaagga aaaccagtcc tggatctcta ggcctaagga 181 gaaaggaact cctcccttct tccatcttct taggggttct aaggaaaagg ctacagggtc 241 tgcagcagac tagggactgt gtggctgata ttgttagccc aggtgcccta aaagaaacca 301 cacagccatc tcccagcctt tgcctggcac caagtgggtt cttggcacca agtgggttct 361 agggagagaa accgtctcac tgaaggggtg ggctacagct ggccgtacat gtccagttcc 421 ctgaaagggg aactgaggat gacagttgtg gaaggtgagg cctcttccca agacgcccac 481 aggcccacct ctctcaaacc cagaccctca tgctggtcag gctctcagat gtacactctt 541 cttagtttca agccgcagag gccagactcg acccgcaggt ctcagaactc tgggggctct 601 gactcagaca agatccttcc agatcgccaa aggacc //