LOCUS BG082923 642 bp mRNA linear EST 12-MAY-2010 DEFINITION H3082D05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3082D05 5', mRNA sequence. ACCESSION BG082923 VERSION BG082923.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 642) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082923.1. Other_ESTs: H3082D05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3082 row: D column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..642 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3082D05-5" /db_xref="taxon:10090" /clone="H3082D05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 188 a 133 c 196 g 125 t ORIGIN 1 ggaacgcgac ggcgagggtg gcagtggcag cgttcgtgtg ctcgggtctg aatcgctgag 61 ggaggaggcg gcggcggagg aagaggtggc agcggtggcg gtggtcgtag cggtggcgga 121 ggaggcgggt acgaatcagc tgcgggcaga gacatggcca acatcgcggt gcagcgaatc 181 aagcgggagt tcaaggaggt gctgaagagc gaggagacga gcaaaaatca aattaaagta 241 gatcttgtag atgagaattt tacagaatta agaggagaaa tagcaggacc tccagacaca 301 ccgtatgaag gtggaagata tcaactagag ataaaaatac cagaaacata tccatttaac 361 ccccctaagg tccggtttat cactaaaata tggcacccta atattagttc cgtcacaggg 421 gctatttgtt tggatatcct gaaagatcaa tgggcagcag caatgactct gcgcacggta 481 ttattgtcat tgcaagcgct gttggcggct gcagaaccag atgaccccca agatgcagta 541 gtagcgaatc agtacaaaca gaatcctgaa atgttcaagc agacagctcg actttgggca 601 cacgtgtacg ctggagcacc agtttctagt ccagaataca cc //