LOCUS BG082753 578 bp mRNA linear EST 12-MAY-2010 DEFINITION H3079H01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3079H01 5', mRNA sequence. ACCESSION BG082753 VERSION BG082753.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 578) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082753.1. Other_ESTs: H3079H01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3079 row: H column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..578 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3079H01-5" /db_xref="taxon:10090" /clone="H3079H01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 167 a 116 c 149 g 146 t ORIGIN 1 gttagcccgg tgcacgatgc ccccgaaaaa gggaggagat ggaattaaac cgcctccaat 61 tattggaaga tttggaacct cactgaaaat tggtatcgtt ggattgccaa atgttgggaa 121 gtctaccttc ttcaatgtat taaccaatag tcaggcttca gcagaaaact tcccattctg 181 tactattgat cctaatgaga gcagagtgcc tgtgccagat gagaggttcg actttctttg 241 ccagtaccat aaaccagcaa gcaagattcc tgctttccta aatgtagtgg atattgctgg 301 ccttgtaaaa ggagctcaca atgggcaggg cttggggaat gcctttttat cccatattag 361 tgcctgtgat gggatttttc atctaacacg tgcttttgaa gacgatgata tcacgcatgt 421 tgaaggaagt gtagatccca tccgagacat agagataatt cacgaggagc tccagctgaa 481 ggacgaggag atgattgggc ccattctaga caaactagag aaggtggccg tgaggggagg 541 cgacaagaaa ctgaagcccg agtatgacat aatgtgca //