LOCUS BG082716 545 bp mRNA linear EST 12-MAY-2010 DEFINITION H3079D06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3079D06 5', mRNA sequence. ACCESSION BG082716 VERSION BG082716.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 545) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082716.1. Other_ESTs: H3079D06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3079 row: D column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..545 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3079D06-5" /db_xref="taxon:10090" /clone="H3079D06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 99 a 166 c 140 g 140 t ORIGIN 1 gagaaatgtt acgaccagct cttcgtgcaa tgggacttgc ttcatgttcc ctgcctcaag 61 attctcctca gcaaaggcct cgggctgggc atcgtggctg ggtcacttct cgtcaagctg 121 ccccaggtat ttaaactctt gggagccaag agtgcagaag gactgagtct ccagtcagta 181 atgctggagc tagtggcact gaccggaacc gtggtctaca gcatcaccaa caacttcccc 241 ttcaggagtc gctttccttg cctgctatgc catggccctg ctggcgctgc tctccccgct 301 cacgcctctg gctgtagtca ctctgctcca ggcctccaat gtacctgccg tggtgggggg 361 gaagttgctt caggcagcca ctaactaccg caacggacac acaggccagc tttcagccat 421 tacagtgttt atgctgtttg ggggctcctt ggcccgaatc ttcacttctg ttcaggaaac 481 tggagacccc ctcatggctg gagtctttgt ggtctcttct ctctgcaatg gcctcattgc 541 tgccc //