LOCUS BG082702 555 bp mRNA linear EST 12-MAY-2010 DEFINITION H3079C04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3079C04 5', mRNA sequence. ACCESSION BG082702 VERSION BG082702.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 555) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082702.1. Other_ESTs: H3079C04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3079 row: C column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..555 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3079C04-5" /db_xref="taxon:10090" /clone="H3079C04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 141 a 145 c 134 g 135 t ORIGIN 1 ctcagactga gcccactcgc taagttcttt ccttttcctt tgtttccagc ccagcaaaag 61 aattacacct tccgctgcct gcagatgtct tcctttgcaa acagaagctg gtcccgcaca 121 gacagcgtgg tctggctggg ggatctgcag actcaccgtt ggagtaatga ctcagccacc 181 atcagcttca cgaagccatg gtcccagggc aagttgagta accagcagtg ggagaagttg 241 cagcatatgt ttcaagtcta tcgagtcagc tttaccaggg acatacagga attagtcaaa 301 atgatgtcac ctaaagaaga ctatcccatt gagatccagc tgtctgcggg ctgtgaaatg 361 taccctggga atgcttcgga aagcttttta cacgtagcat ttcaaggaaa atatgtcgtg 421 agattctggg gaacgtcctg gcagacagtc ccaggggccc catcttggtt agacttgccc 481 atcaaagtgc tcaacgctga tcaagggaca agtgcaaccg tgcagatgct cctgaatgac 541 acctgccccc tattt //