LOCUS BG082521 518 bp mRNA linear EST 12-MAY-2010 DEFINITION H3077B02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3077B02 5', mRNA sequence. ACCESSION BG082521 VERSION BG082521.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 518) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG082521.1. Other_ESTs: H3077B02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3077 row: B column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..518 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3077B02-5" /db_xref="taxon:10090" /clone="H3077B02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 138 a 116 c 116 g 148 t ORIGIN 1 cgacagatca ggttaaatga attattaaag gagcattcaa gcacagctaa cattattgtc 61 atgagtcttc cagtcgcacg gaaaggcgct gtgtccagtg ctctgcatat ggcttggtta 121 gaagctctct cgaaggacct gccaccagtc ctcctggttc gtgggaatca tcagagcgtc 181 cttaccttct actcataact gttctgcacc atggacttcc tctaaggatg gcacttcagt 241 gcccagtcaa agtgactgta tcgtcaggaa caccacagca tcgcagtggc agacggggac 301 tgcttcacta cttcattatt ggaaagcaca tggaaagttg ccccactgat agatagatgg 361 agacttcagt tgtagtctac tccagatctc aatcttaatg actgctaatt ctttcttgtt 421 ggactgaagt tcatgagagt aaaattttcc tttgctactt gaaagcaata agagtgagtt 481 attttttgat tgctgaaagg agtgcaaagc ctttagcc //