LOCUS BG082157 645 bp mRNA linear EST 26-JAN-2001
DEFINITION H3073B03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3073B03 5', mRNA sequence.
ACCESSION BG082157
VERSION BG082157.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 645)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3073B03-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3073 row: B column: 03
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..645
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3073B03-5"
/db_xref="taxon:10090"
/clone="H3073B03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 177 a 130 c 138 g 200 t
ORIGIN
1 actcccaatg acggtgatgt ataatgctca agtattctcc tgctttttta ccactaactg
61 ggaactgggt ttggccttaa ttcagacagc cttggttctg tctggacagg tccagatgac
121 tgacaccatt aacactttgt cagcctcagt gactacagtc atagatgaac aggcctcagc
181 taatgtcaag atacagagag gtctcatgct ggttaatcaa ctcatagatc ttgtccagaa
241 acaactagat gtattatgac aaataactca gcagggatgt gaacaaaagt ttccgggatt
301 gtgtgttatt tccattcagt atgttaaatt tactagggca gctaatttgt caaaaagtct
361 ttttcagtat atgttacaga tccttcgaga attgagactt caggtcaact ccacgcgctt
421 ggacctgtcg ctgaccaaag gattacccaa ttggatctcc tcagcatttt ctttctttaa
481 aaaattgggt gggattaata ttatttggag atacactttg ctgtggatta gtgttgcttc
541 tttgattggt ctgtaagctt aaggcccaaa ctaagagaga caaggtggtt attgcccagg
601 cgcttgcagg actagaacat ggagcttccc ctgatatatc tatgc
//