LOCUS BG081526 661 bp mRNA linear EST 12-MAY-2010 DEFINITION H3066B12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3066B12 5', mRNA sequence. ACCESSION BG081526 VERSION BG081526.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 661) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG081526.1. Other_ESTs: H3066B12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3066 row: B column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..661 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3066B12-5" /db_xref="taxon:10090" /clone="H3066B12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 180 a 170 c 158 g 153 t ORIGIN 1 attcacgatt gttggtgacg cagttggctg gggctttgtg gtacgaggaa gtaaaccatg 61 ccacatccag gctgtggacc ccagtggacc tgcagccgct gcagggatga aggtctgtca 121 gtttgtcgtc tctgtcaatg gcctcaacgt gctgaatgta gactaccgca ccgtgagcaa 181 tctgattctg acaggcccaa ggacaattgt catggaagtc atggaggagt tggactgctg 241 agcacagaga catccctgct tgtcccctat cccagaacaa cacaacagtg gcgaggcaag 301 gctgccaggc acatggataa ttgggacatt gcagatcttt tctctgatca tatgcacctt 361 gtctttcata cgctgtttga atgtggaaga accaggttac atatctttaa aaaccaaaac 421 acagcagggg cttggtggct caagtctata atcccagcat tcaggagggc aaggcagaag 481 tgctgcaaat tcaaggcaag cgtctactaa cagtgtgagt gcctgtctca aaaaccacac 541 tcgggatctt tccacacaag tgcacatacc acacacatgc acacacacac acatgcacac 601 cacacacaca cgcaccaaag cccagtttag agttctcaat cattgttctt catgttttcg 661 g //