LOCUS BG081506 498 bp mRNA linear EST 12-MAY-2010 DEFINITION H3066A03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3066A03 5', mRNA sequence. ACCESSION BG081506 VERSION BG081506.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 498) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG081506.1. Other_ESTs: H3066A03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3066 row: A column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..498 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3066A03-5" /db_xref="taxon:10090" /clone="H3066A03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 142 a 128 c 151 g 77 t ORIGIN 1 atttggaaaa tgagcatgct tgggtcaggg agagattcta ccaggacaag ctgcggtggg 61 acaagtgcag gtattaccac gacaggtaca cgcccctata cacggcccgg gacgcccgag 121 aatggcggcc tctgcatggt cgtgagcatg accgccttgt ccagtctgga cggccataca 181 aggacagcta ctggggccgc aagggctggg agctgcaatc ccgggggaag gaacggcccc 241 acttcaacag cccccgagag gcccctagcc ttgctgtgcc cctcgagaga catctccaag 301 agaaggctgc gctgagtgtg caggacagca gccacagtct ccctgagcgc tttcatgaac 361 acaaaagtgt caagtcgagg aagcggaggt atgagactct agaaaataat gatggccgtc 421 tagaaaagaa agtccacaaa agcctggaga aggacacgct agaggagcca agggtgaaga 481 agcacaaaaa gtctaaaa //