LOCUS BG081227 660 bp mRNA linear EST 12-MAY-2010 DEFINITION H3062G03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3062G03 5', mRNA sequence. ACCESSION BG081227 VERSION BG081227.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 660) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG081227.1. Other_ESTs: H3062G03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3062 row: G column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..660 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3062G03-5" /db_xref="taxon:10090" /clone="H3062G03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 186 a 160 c 145 g 169 t ORIGIN 1 caacaaacaa acaggaggaa aggcaaacaa accaacagca aaacaaaaac aaaaggaaac 61 aacttaaggc agagtggcga aaataatccc taaatctgct gcctgctcct ctttcctgcc 121 aatccgagca cactgctgaa caggaagagt ttcgaagaga ctgtgaatcc cagacagagt 181 acaggaagtg caaggccagt gtctgagtca ttgtaccaag cctgccgccc acaggtagca 241 cctcggtctc cctgtgcagt tggagccctg cagcctgtcc caggctgttt gtggtttgca 301 tatctgtcct agttatactg cttaggttct gtgtgtgaaa ctgtgacttc atgtgtcact 361 ttttcatgtc ctctgaagct atttttaaga aagatctctg ccaaacaaac ccattgttcc 421 atctgcattc agggttttag aaggattttt agtttcatcc aattggcaca attgtgccag 481 tgcgttctgg caccctttgg aagtaaagca gttatgggca aagagtcgcc aagaacaaac 541 gccactcttg tttcacatac agccaattac atcacaggag tgctgacttc cgaggcagtg 601 ggcaagtgta gaatgctgag cccctttgac ttgattcatg ccttaatccg tactatttca //