LOCUS BG081185 778 bp mRNA linear EST 26-JAN-2001
DEFINITION H3062A11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3062A11 5', mRNA sequence.
ACCESSION BG081185
VERSION BG081185.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 778)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3062A11-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3062 row: A column: 11
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..778
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3062A11-5"
/db_xref="taxon:10090"
/clone="H3062A11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 268 a 143 c 117 g 247 t
ORIGIN
1 ttggaaatgt aaatgagcta aatacctaat aaaaaatgga aaaaaaatca atattagaag
61 tgttactatc atgttacaaa gtttggctgc caatttgtac ctatgaatgc atgacagttc
121 aaattttaat gcttcattaa agagacattg ttttaaattt tatctctaag tccacatttt
181 aggataagaa tcacattaaa tattatctca actagaagta tccttagagg cctagttttt
241 tttgcctgct ttatgccacg tgtttgaaag actccaaacc ggatttacca atttaaagct
301 gtcttgatac caacgttaca cccttttaaa tcatacccag taacttataa gctaatattc
361 tatgatgaag acacgtagaa catagtatac ctttaggacc agtaaaaacc aaactaagtg
421 gctacaggaa tcctataaat ttaggccagt caaagaatat tactttttct agctatattt
481 ataagataaa aaagcccctt tttttcattt gctaaacaca ataatcacga ttgtggagaa
541 ttttctagga aaaaactaac tatcagcttt tttgccttaa aactaagaag ctgcagactc
601 cttttctttc aaagcagttt ttccagcagt ggctcccctg ctgtgcttga ttcttggata
661 aagtgtggac cactcttcgg ctttgctgaa ccatcagata aagtcttagc cattnttatt
721 ttccaaaatg aaacacagaa cattcagtca ttcagacana aaaaaaaagg gcggncgc
//