LOCUS BG080989 603 bp mRNA linear EST 12-MAY-2010 DEFINITION H3059G10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3059G10 5', mRNA sequence. ACCESSION BG080989 VERSION BG080989.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 603) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080989.1. Other_ESTs: H3059G10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3059 row: G column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..603 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3059G10-5" /db_xref="taxon:10090" /clone="H3059G10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 132 a 145 c 183 g 143 t ORIGIN 1 ggcttcggca ggaatggagc cgctggacga gctggacctg ctgctgctgg aagaggacgg 61 cggagcggag gccgtgccgc gggtggagct acttcggaag aaggcggacg ctttgtttcc 121 agagacagtt ctgagccgag gagtggataa ccggtacctg gtgctagctg ttgagacctc 181 gcagaatgaa agaggagccg aggagaagcg cttgcacgtc acggcgtccc aggaccgaga 241 gcacgaagtt ctgtgcatcc tgaggaatgg ctggtgattt gcatttttga ctttgagcca 301 tgatttggtg gtctggtggt ttgttgtttt tgtttgctgt cacgtagccc aggctgtacc 361 tgaactgatg gtatctcgcc tccacttcct tacctccaaa tgctgcgatt acctggcatg 421 atttggtagc gtctggagga caccacctga cctaaggcct tgagtgtgcc aggcaaacta 481 agctccaccc ctaagttccc gtcccaactc taggacatca cctgaaaacc catcttgcgt 541 ggataaatta tttgaaagga ggtagagatc gttcaagggt aaaggtgcct gctacccaaa 601 ctg //