LOCUS BG080920 605 bp mRNA linear EST 12-MAY-2010 DEFINITION H3059A07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3059A07 5', mRNA sequence. ACCESSION BG080920 VERSION BG080920.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 605) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080920.1. Other_ESTs: H3059A07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3059 row: A column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..605 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3059A07-5" /db_xref="taxon:10090" /clone="H3059A07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 127 a 157 c 191 g 130 t ORIGIN 1 cgcaaagatg acgcttgccg tcctggccct gcggctggtg gtctgtaccc tggcgcttcc 61 catgtttctg ctgaaccttc taggcttgtg gagctgggta tgcaaaaagt gctttcccta 121 cttcctgaaa cgattctccg tgatgtacaa tgagcagatg gcgagccaaa agcgggagct 181 cttcagcaat ctgcaggagt tcgccggccc ctcggggaag ctgactctgc tggaggtggg 241 ctgcggcacc ggagccaact tcaagttcta tccccccggg tgcagggtca cttgtatcga 301 ccccaacccc aacttcgaga agttcttgtt caagagcgtc gcagagaacc ggcagctgca 361 gttcgagcgc ttcgtggtgg cagccgggga ggacatgcac caggtgaccg atggctctgt 421 ggacgtggtg gtctgcaccc tggtgctgtg ctcggtgaag aaccaggaga agattctgcg 481 tgaggtgtgc cgagtgctga agccggtgag tggcaggagt gaggactggc tagctgcagc 541 taggagtgag ggtgctccta tcagtttcgg gtggattcta aaccaaaaca aacaaacaaa 601 caaac //