LOCUS BG080899 687 bp mRNA linear EST 12-MAY-2010 DEFINITION H3058G08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3058G08 5', mRNA sequence. ACCESSION BG080899 VERSION BG080899.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 687) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080899.1. Other_ESTs: H3058G08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3058 row: G column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..687 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3058G08-5" /db_xref="taxon:10090" /clone="H3058G08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 226 a 140 c 142 g 179 t ORIGIN 1 ccactactga agatgaagag caaaccagat caggagctcc atggttcaaa aagcctttaa 61 gaaatacctc agttgtagac agtgagccag tcagatttac tgttaaagta acaggagaac 121 ccaagccgga aattacatgg tggtttgaag gagaaatact gcaggatgga gaagactatc 181 agtacatcga aagaggtgaa acttactgcc tgtatttacc ggaaaccttc ccagaagatg 241 gaggagagta catgtgtaag gcagtcaaca ataaaggctc agcagcgagc acctgcattc 301 ttaccattga aagtaagaac taattggctt tttgatcctt tgttttatta ggaaataaat 361 aggaaaaaaa atcatccttc tccttcttct tttctagtgg atgactacta ggcttccctc 421 tgtccttggg actctctctc tcgctgcatc tctgtggagg ggccaaaaag gagaccagag 481 gtgccactat aactgactta atctttcccc aaatcttcct cttaagaact tctcatgcat 541 atcaggttca ttaccatgct gtgcaaagtc aaagcatagc tgacagaaaa gggaaataaa 601 tgtacccatt ctgtcagaac taagacagaa gcttcgtatt tatagaacta agacttaaca 661 tatacagttt gcatgaataa actggag //