LOCUS BG080791 541 bp mRNA linear EST 12-MAY-2010 DEFINITION H3057E10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3057E10 5', mRNA sequence. ACCESSION BG080791 VERSION BG080791.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 541) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080791.1. Other_ESTs: H3057E10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3057 row: E column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..541 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3057E10-5" /db_xref="taxon:10090" /clone="H3057E10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 136 a 129 c 163 g 113 t ORIGIN 1 gcatggagtt ccagggcgag cgcgggaccg gtcctggtgt ttcttcgtct tctgtagcct 61 gctcgcaggt cacggtgtcc agggagctgc tgacggcggg gagcgagggt tcgggaggta 121 tctgggacca gttgctcatc agctctaagc cccatcccag aaagacctct acacttcaaa 181 cagttcggat gcagaggagc cctttgctag accaggttca ggccttcctc ccacaaatgg 241 cccaggcaaa tgagaagctg aggagggaaa tggcagctgc tccagctggt cacttcaata 301 ttgaaaacat cgacgagacg tctggaaaca tcatacagat ggatgtggcc ttatttgaga 361 tgagtcggtc agattcaaaa gaggaggata gcccagaaga gagctctcgg gacagttctg 421 gggacagctc ggagtccgaa gaagatgtct gcgttccctc tgaagtcacc atcgagaaca 481 ttaagcttcc gatgcagaag gtggcaaagg caagattgaa attttggaca gtccggcaag 541 t //