LOCUS BG080578 518 bp mRNA linear EST 12-MAY-2010 DEFINITION H3055C05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3055C05 5', mRNA sequence. ACCESSION BG080578 VERSION BG080578.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 518) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080578.1. Other_ESTs: H3055C05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3055 row: C column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..518 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3055C05-5" /db_xref="taxon:10090" /clone="H3055C05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 122 a 151 c 128 g 117 t ORIGIN 1 acatcgatgc tcaccctgag accatcgtct tggatcccct ccccgccatc aggaccctgt 61 tagaccgttc caagtcctac gaacttatcc gaaagatcga ggcctacatg aaaggtgaca 121 gaatctgctc gccgcctttc atggagctca cgagcctgtg tggggaggac accatgaggc 181 tgctggagca gaacggcctg gccttcccct tcatttgcaa aaccagagtg gctcatggaa 241 ccaactctca tgagatggct attgtgttca accaagaggg cctgaatgcc atccagcctc 301 cctgtgtggt ccagaacttc atcaaccaca atgctgtcct gtacaaggtg tttgtggtgg 361 gcgagtccta cacagtggtc cagagaccct cactcaagaa cttctctgcg ggcacatcag 421 atcgtgagtc catcttcttc aacagccaca atgtgtcaaa gccggagtct tcatcagtcc 481 tcactgagct ggacaagatc gaggggtgtg ttcgaacg //