LOCUS BG080543 618 bp mRNA linear EST 26-JAN-2001 DEFINITION H3054H04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3054H04 5', mRNA sequence. ACCESSION BG080543 VERSION BG080543.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 618) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3054H04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3054 row: H column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..618 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3054H04-5" /db_xref="taxon:10090" /clone="H3054H04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 109 a 183 c 192 g 134 t ORIGIN 1 gggtttcgta cgtagcagag cagctccctc gctgcgatct attgaaagtc agccctcgac 61 acaagggttt gtctacgaat ggtttagcgc caggttccac acgaacgtgc gttcaacgtg 121 acggggatag tcgactatcc ggcgaaggcg aggcgttgct gtccctggcc atgctcctgc 181 gtctctacct ggtgccccgc gcggtgctgc tgcgcagcgg ggtcctgctc aacgcgtcct 241 accgcagcat cggggcgctc aaccaagtcc gcttccgcca ctggttcgtg gccaagctgt 301 acatgaacac gcacccgggt cgcctgctgc tgggcctcac gctgggtctc tggctcacca 361 cagcttgggt gctgtctgtg gctgagaggc aggctgtcaa tgccacgggg cacctcacag 421 acacactgtg gctgattccg atcacattcc tgaccattgg ctatggggac gtggtacctg 481 gcaccatgtg gggcaagatt gtctgcctgt caccggagtc atgggggtct gctgcacagc 541 tctcctggtg gctgtggtgg ctcggaagct ggagttcaac aaggcggaga aacacgtgca 601 caacttcatg atggacat //