LOCUS BG080484 650 bp mRNA linear EST 12-MAY-2010 DEFINITION H3054C01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3054C01 5', mRNA sequence. ACCESSION BG080484 VERSION BG080484.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 650) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080484.1. Other_ESTs: H3054C01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3054 row: C column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..650 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3054C01-5" /db_xref="taxon:10090" /clone="H3054C01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 158 a 201 c 141 g 150 t ORIGIN 1 ggcctggggc acaagcaagc cagccatctg cccagacctt tccaggacca cgacagactc 61 tttacctcca tgggttgcct ccacgggttg ctacagcctg gacatggaaa cctgtgagat 121 ctctccactg tttttttaag ctcccatctt ccggcagtga atggcacacc acatgagtca 181 acgtgtcaga acggatgtta aggagagggc gcgagtgctc aaactcaagg attccagctg 241 aacggatgct aacatcttcc tggcttctgc tgtaacctct gaactactga gactcgagat 301 acttcagtgc tgtgtacagt ctgcctctgc gttccctacc ccctgccaga agttccaatc 361 ctccctccag cccgcaacaa gcatgataca cagacaactg gtttctgtgc tgtgtcctcc 421 tgcagcaagc ccagggctct ctgccccttt cctggccgct cccctagtgt taaaatggca 481 ctatccataa aactcctcct taatgaggcc attctgagga gacagcacga gacatcctcc 541 atgcctttgt gtctccaaaa aaaaccaaca ctcctcctga taagcagaac tctccaggtg 601 cctgtccttg agaccttaga gaaataacaa gctcaaggga gactagggcc //