LOCUS BG080462 651 bp mRNA linear EST 12-MAY-2010 DEFINITION H3054A01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3054A01 5', mRNA sequence. ACCESSION BG080462 VERSION BG080462.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 651) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080462.1. Other_ESTs: H3054A01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3054 row: A column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..651 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3054A01-5" /db_xref="taxon:10090" /clone="H3054A01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 179 a 175 c 173 g 124 t ORIGIN 1 attgacgtcg cctatccaga aaaagatgaa gcttgagaga tgaagaaact gctgttagaa 61 gaactggtgg tcgtgttgtt cttgttggtc gagagcagac gcgacaggta gaagcatcca 121 tcaggcagaa aatgcagtct tcctgagtgg agaactcgag ccgaacgctt gtttcctgct 181 ccagactgga tatgcctctt catgtccgcc ggagcagcga cccagcttta actggccttt 241 ccacttctgt cagtgataac aacttttcct cagaggagcc ctccaggaaa aaccccaccc 301 gctggtccac gacagctggc tttctcaagc agaacaccgc tggaagtccc aaaacctgcg 361 acaggaagaa agatgaaaac tacagaagcc ttccacggga tcccagtagc tggtccaacc 421 agttccagcg agacaacgcc cgctcctccc tgagcgccag ccacccaatg gtagaccggt 481 ggctggagaa gcaagaacag gatgaggaag gcacagaaga agacagcagc cgagtggagc 541 cggttggaca tgctgatacc ggattggaga acatgcccaa cttttccctc gatgatatgg 601 taaagctcgt acaagtcccc aacgatggag ggcccctggg aatccatgta g //