LOCUS BG080424 661 bp mRNA linear EST 12-MAY-2010 DEFINITION H3053E02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3053E02 5', mRNA sequence. ACCESSION BG080424 VERSION BG080424.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 661) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080424.1. Other_ESTs: H3053E02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3053 row: E column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..661 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3053E02-5" /db_xref="taxon:10090" /clone="H3053E02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 163 a 209 c 174 g 115 t ORIGIN 1 catggactat gagcgggctc tccggctcct caggttgccc cagctacccc ctggtgccca 61 gctggtgaag tccacctggc tgagcttgtg cctgcaggag ggaaggctga cagacacgga 121 aggattcagc cttcccatgc ccaagaggtc cttggatgaa ccacagccca gcaagtcagg 181 ccaagatgct tctgctcctg gcacccagcg ggatttaccc aggaccaccc tttctctttc 241 ccctcctcac accagagctg tatctcctcc cccaacggca gaaaagccat caagaaccca 301 agcccagctc agctcagagg atgaaaccag cgatggagaa gggccccagg ttagctcagc 361 agatctgcaa gccttgatca ctgggcacta ccccactccc cctgaggaag atggtgggcc 421 tgacccagca ccagaagctc tggataaatg ggtctgtgca cagccctcga gccagaaggc 481 aactaactac aatctgcaca tcacagagaa gctggaagtg ctggctaaag cctacagtgt 541 ccagggagac aagtggaggg ccctgggcta tgcaaaggcc atcaatgctc tcaagagctt 601 ccacaagcct gtcaactcct accaggaggc ctgtagcatc ccgggaattg gcaagcggat 661 g //