LOCUS BG080405 644 bp mRNA linear EST 12-MAY-2010 DEFINITION H3053C05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3053C05 5', mRNA sequence. ACCESSION BG080405 VERSION BG080405.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 644) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080405.1. Other_ESTs: H3053C05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3053 row: C column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..644 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3053C05-5" /db_xref="taxon:10090" /clone="H3053C05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 105 a 188 c 182 g 169 t ORIGIN 1 ctgtggtccg atttaccttc gggggactgc aggccttggg gacgctcctc tatagctgct 61 actctggctt ggagagctta aagctactgg ggcacttggc ctcccacgga gctctgagga 121 gccgggaatt cctgaatcgg ggcatcctga acatggtctc caatggccat gctttgctgc 181 gccaggcctg tgacatatgt gctattgcca tgagcctggt ggcctatgtg atcaacagtc 241 tagtcaacat ctgcctcatc agcactcaga acttcttctc cctggtgctg gccctgtggg 301 atgctgtaac ggggcctctt tggaggatga cagacgtggt ggctgctttc ctcgctcaca 361 tctccagcag tgcagtggct atggccattc tcctgtggac cccctgccag ctagcactgg 421 agctgttggc ctcagccgcc cgcctgctgg ccagctgtgt ggttttccat ctcactggat 481 tggtgttgct ggcttgcgtg ctggcagtga ttttgattgt gttgcaccca gaacaaaccc 541 tgaggctggc cactcaagct ctcagtcagc ttcatgcccg cccatcttac caccggcttt 601 gggaggatat cgtacggctg actcgccttc cactaggtct ggag //