LOCUS BG080334 647 bp mRNA linear EST 12-MAY-2010 DEFINITION H3052D06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3052D06 5', mRNA sequence. ACCESSION BG080334 VERSION BG080334.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 647) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080334.1. Other_ESTs: H3052D06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3052 row: D column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..647 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3052D06-5" /db_xref="taxon:10090" /clone="H3052D06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 181 a 143 c 141 g 182 t ORIGIN 1 cttgcatctt cttcgcagac cttgacttca ctgagagagg catagcctag aacttctcct 61 ggtgttcctg ctggtcctaa tcactcctgc tgacttgtac caatttagct gaggcctggc 121 tgtttctgct aggtcatgcc actgctgctg attcatgttt gctatcctga cactattgag 181 cagactgctg gtatgttcac aaagtgtttg caagtggatt gagctgctgc tgctgcttac 241 tatgaactga actgctgatt tcctaacaat acagatggga tttgctccaa agaacaacta 301 aacaggaatg cttcgtggca tctccattga tttgtgagta atcagaaatg caaatagctc 361 ctcaggaatc aagctacgct caaagaaacc ttacttacca atttcaagga gtataccatg 421 gagtgcaaga ctaaggggaa acaccagcat agcttgaact tactagataa aattaagaat 481 atgaaagagt tagaagaaat gattgatgta gtgctcatag cagaagagga gaaattccct 541 tgccacagac tggtgctcgc tgcctttagt ccttatttca aggctatgtt cacctgtgga 601 ctactcgagt gcactcagag agaggtcata ctctatgaca tcacagc //