LOCUS BG080073 624 bp mRNA linear EST 12-MAY-2010 DEFINITION H3049B12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3049B12 5', mRNA sequence. ACCESSION BG080073 VERSION BG080073.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 624) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080073.1. Other_ESTs: H3049B12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3049 row: B column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..624 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3049B12-5" /db_xref="taxon:10090" /clone="H3049B12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 183 a 171 c 122 g 148 t ORIGIN 1 tccctgtgag ttccaggcca gcttgggtta cacagtgaga ctccgtccag cagtgagact 61 ccacccagta gtgagactcc atccagcccc atcccggttt gctttgtctc actgtgacta 121 acaccacgag cagaatcaac ttaagcaaga aaaggcttat ttggctttat gagtcctgat 181 tatagttcca tcaccgaggg aagctagtca ggtcaggtag tgcaaaactg cctgtaactc 241 cagctcccgg ggatctgaca ccctctgttg gttcctgtgg gcccacgtac ccacttgatg 301 tacacacaag gagaggcaca tattcatata cgtaattgaa atataaaata tgtcttttct 361 ttttaaagtc attacctgag tgatagaaag gaaaggactg accccccccc agtgtcttcc 421 acacacatgt atgtagacac acacacacac acacacacac acacacacac acacacacta 481 catacactaa ataaataata ttttaacagc tataacaact ctccatgctg ggtcacacat 541 gtcatccggc acccaggagg ctggaacagg atgttcatga gttggagctg tataatgact 601 tccagcacag caagaccctg tctc //