LOCUS BG080019 630 bp mRNA linear EST 12-MAY-2010 DEFINITION H3047H03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3047H03 5', mRNA sequence. ACCESSION BG080019 VERSION BG080019.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 630) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080019.1. Other_ESTs: H3047H03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3047 row: H column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..630 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3047H03-5" /db_xref="taxon:10090" /clone="H3047H03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 168 a 140 c 144 g 178 t ORIGIN 1 agatccccat gccagaaccg gagcgctccc agtatcccat ggagtggggc aagactttcc 61 tggcctttct ttatgcactt tcctgttttg ttctcactac agtgatgatc tcggtcgtcc 121 atgaacgagt acctcctaag gaggtgcagc ctccactacc ggacacgttt tttgaccatt 181 ttaaccgggt gcagtgggcg ttttctattt gcgaaattaa cggcatgatc cttgtaggac 241 tctggctatt tcagtggctg cccttaaaat acaagtaagt agagtaaaca ctcttgagat 301 ccagccatga aggggtttgc acacagggat ccagttattg gaaatgaaat ttaccaagtg 361 ttgaaatcaa agcttaggaa ttccctttcc tttccattct gaagccacac acccatggaa 421 ctaattgaag ctcctacagc tttgagagga tgatgtattt gttgcctgtt cgctacaaga 481 ttgctccaga attaagacca tgagcaatgc tagtctattt tcaagcagta ggaaagaaaa 541 cacttagaca tcttcagaga gctgaggctg ttgagtaaca gagggggaaa cacctaccta 601 gtcttgaatg gtgtttactc aggtgaattg //