LOCUS BG079934 612 bp mRNA linear EST 12-MAY-2010 DEFINITION H3046F11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3046F11 5', mRNA sequence. ACCESSION BG079934 VERSION BG079934.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 612) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079934.1. Other_ESTs: H3046F11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3046 row: F column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..612 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3046F11-5" /db_xref="taxon:10090" /clone="H3046F11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 151 a 149 c 171 g 141 t ORIGIN 1 gcagccgggt agagaggagc gtggccttct cctctccccg tcatggcttg tgcccgtccc 61 ctcatatcgg tgtactccga aaagggagag tcatctggca agaatgtcac tttgccagct 121 gtgttcaaag ctcccattcg accagatatt gtgaacttcg ttcacaccaa cttgaggaaa 181 aacaacagac agccctatgc cgtcagtgaa ttggcaggtc atcagaccag tgctgagtct 241 tggggtactg gcagagctgt ggctcgaatt ccaagagttc gaggtggtgg gacccaccga 301 tctggccagg gtgcctttgg aaatatgtgt cgtgggggac gcatggttgc accaaccaaa 361 acctggcgtc gttggcaccg cagagtgaat acaacccaaa aacgatatgc catctgttct 421 gccctggctg cctcggcctt accagctttg gtgatgtcta aaggtcatcg tattgaggag 481 gttcctgagc tgccgctggt ggttgaagat aaggttgaag gttacaagaa gaccaaggag 541 gctgttcagc tgctcaagaa actcaaagct tggaatgaca tcaaaaaggt ctatgcctct 601 cagagaatga ga //