LOCUS BG079888 589 bp mRNA linear EST 12-MAY-2010
DEFINITION H3046C09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3046C09 5', mRNA sequence.
ACCESSION BG079888
VERSION BG079888.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 589)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG079888.1.
Other_ESTs: H3046C09-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3046 row: C column: 09
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..589
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3046C09-5"
/db_xref="taxon:10090"
/clone="H3046C09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 182 a 126 c 123 g 158 t
ORIGIN
1 ctggcaaaga ggttgtcact agtcttcatc tacaggagag tgggagcagt gtgctacatt
61 accaaagaca gacatgccat gtgcacactt atgaataggg gatgggagta caggggattc
121 atatttctac aaacaagtag aatagagtcc ctactatgac aggcctgggc ccagaacacg
181 tcttcccact cagcacatag cctagcttgt agagtccacc caacaacctc cacagagctg
241 gggttcttat gccctaactt tagttaagac ctagttgcca cagaatctca atttatctca
301 gctttgtgat atgcagaaat ttggatgata attaaagctg aaaaacatgg cccataagaa
361 ctacatataa agaactacac ataaagaact acatacgcct gctgccaagc ctggatggca
421 tgaacttgat ctttaggacc tacattgcag gagaaaaaca attctgattt atttatttat
481 ttatttattt atttaatgta tgtgagtaca ctgtcactct cttgggacac accagaagag
541 ggcatctgat cccattacag atggttgtga gccaccatgt ggttgctga
//