LOCUS BG079875 603 bp mRNA linear EST 12-MAY-2010 DEFINITION H3046B07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3046B07 5', mRNA sequence. ACCESSION BG079875 VERSION BG079875.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 603) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079875.1. Other_ESTs: H3046B07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3046 row: B column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..603 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3046B07-5" /db_xref="taxon:10090" /clone="H3046B07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 221 a 107 c 168 g 107 t ORIGIN 1 ataagaaata gaattaagca gatgatggac gtctctcgga cacaaactgc agtctcaata 61 gttgaagagg atctaaaact tttacagctt aagctaagag catcgatgtc cactaaatgt 121 aacctggaag accaaatcaa gaaattagaa gatgaccgca gctcgctgca gactgctaaa 181 gctgggttgg aagacgagtg caaaactctg aggcagaaag tggagatcct gaatgagctg 241 taccagcaga aggagatggc gctgcagaag aaactgagtc aagaagagta tgagcggcaa 301 gacagagagc agaggctcac ggcggccgat gagaaggtgg ttctggctgc agaggaagtg 361 aaaacgtaca agcggcgaat cgaagaaatg gaagaagaat tacagaaaac agaacgttca 421 tttaaaaacc agattgctgc tcatgagaag aaagctcatg ataattggct caaagctcgt 481 gctgcagaga gagccatggc agaggagaag agagaagctg ctaacttaag gcacaaatta 541 ctggaaatga ctcaaaagat ggcaatgagg caagatgagc ccgtgattgt aaagccgatg 601 cca //