LOCUS BG079795 604 bp mRNA linear EST 12-MAY-2010 DEFINITION H3045B07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3045B07 5', mRNA sequence. ACCESSION BG079795 VERSION BG079795.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 604) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079795.1. Other_ESTs: H3045B07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3045 row: B column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..604 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3045B07-5" /db_xref="taxon:10090" /clone="H3045B07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 92 a 191 c 156 g 165 t ORIGIN 1 ggaccacccc gctgttatca agcggcgttc caccagtgtc ctggtagtgt ccagcttgtc 61 ccctctttgc gtgctgctct ggagggaact cactggcatc cagccaggca catcactgct 121 taccttgatg ggcttcaggc tggagggcat tttcccagca gcgctgctgc ccctgctgct 181 aactatgatc cttttcctgg gtccactgat gcagctctct atggattgcc cttgtgacct 241 gacagatggg ctgaaggttg tcctggcccc tcgttcttgg gcccgctgcc tcacagacat 301 gcgctggcta cgaaaccaag ttattgcacc gctgacagag gagctggtgt tccgggcttg 361 catgctgccc atgctagcgc cgtgcacggg tctgggccct gctgtgttca cctgcccact 421 cttttttgga gtcgcccatt ttcaccacat tattgagcag ctgcgcttcc gccagagcag 481 tgtgggaagt atcttcgtgt ctgcagcgtt ccagttctcc tacaccgctg tcttcggtgc 541 ttatacagct ttcctcttca tccgcacagg acacctgata gggccggttc tctgccactc 601 tttc //