LOCUS       BG079500                 580 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3041B08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3041B08 5', mRNA sequence.
ACCESSION   BG079500
VERSION     BG079500.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG079500.1.
            Other_ESTs: H3041B08-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3041  row: B  column: 08
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3041B08-5"
                     /db_xref="taxon:10090"
                     /clone="H3041B08"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          137 a          133 c          171 g          139 t
ORIGIN      
        1 cagatgcctt ccaggacgaa ctccaggaac ttggcattcc ccaagatatg attggagatc
       61 tggccagttt ggcatttggg agtcaacgcc ctcttctcga ctctgtagcc cagcagcagg
      121 gatcctcgct gccttttgtg tcgaatttcc ggtggcgggt ggatgtggcc atctcaacca
      181 gtgctcagtc ccgctccctg caaccgagtg ttctcatgca gctgaagctc acagatggat
      241 cggcacaccg cttcgaggtg cccatagcca aattccagga gctgctctac agcgtggcgt
      301 tggtccttaa ggagatggca gagctggaga gaaagtgtga gcgcaaactg caggactgaa
      361 ccctggtact gtggggtgct gaagctggta ccagaaccca gccgcactgg tgatgaggat
      421 gtaagaacat attttaagtg aaaagacagc tgggactttt gggttttgtt ttacaagtaa
      481 acagctgggc tgaggctgga gaatgtgaaa tgtaaggccg gcctggctgg gttttacgta
      541 gtgaggctca gtttcgaatt aaagaggtaa agcaactatt
//