LOCUS BG079273 607 bp mRNA linear EST 12-MAY-2010 DEFINITION H3037H02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3037H02 5', mRNA sequence. ACCESSION BG079273 VERSION BG079273.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 607) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079273.1. Other_ESTs: H3037H02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3037 row: H column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..607 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3037H02-5" /db_xref="taxon:10090" /clone="H3037H02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 133 c 161 g 152 t ORIGIN 1 ggatagagcc tatccaggtg cctcttgagg aggatggaga acagactcag atttgtcaaa 61 gcagagtgtg tgctgacagg gtaaatactt atgattgtgg agaaaatgtt tcaagatggc 121 tgtcaaagtt tcttggccgt ctatgtcatt taatcaagca aagtccacac ttccagcgaa 181 atgccaggaa gacgcctaaa aaaggtcagc ctcccggtac tactgttgcc ctgtccttgg 241 tgaacgaggc tcagtatctg ctggtgaaca catcaagtat tctggagctt cagcggcagt 301 taaatgccag tgacgaacat ggaaaggagg agtcattctc catgaaagat ctcatctccc 361 gcttccgtgc caatattatt accaagggag ccagagcctt tgaagaagag aagtgggatg 421 agatttcaat tggctccttg catttccagg ttctagggcc ttgccacagg tgccagatga 481 tttgtatcaa ccagcaaacc gggcagcgga accaggacgt cttccagacg ctctccgaga 541 gtcgggggag aaaggtgaac tttggtgtgt acctgatgca ttcgtacttg gatttatcgt 601 ctccatg //