LOCUS BG078870 661 bp mRNA linear EST 12-MAY-2010 DEFINITION H3033A07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3033A07 5', mRNA sequence. ACCESSION BG078870 VERSION BG078870.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 661) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG078870.1. Other_ESTs: H3033A07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3033 row: A column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..661 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3033A07-5" /db_xref="taxon:10090" /clone="H3033A07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 188 a 148 c 192 g 133 t ORIGIN 1 gtcagagaag gagccaacta cggtggagac aaagaagcag ctttgcccct atgctgcagt 61 gggagagtgt cgctatgggg aaaactgtgt gtatctccac ggagactcgt gtgacatgtg 121 tggcctgcag gtcctacacc cagtggatgc tgcccagagg tcacagcaca taaaatcttg 181 cattgaggcc catgagaagg acatggagct ctcttttgct gttcagcgca gcaaggacat 241 ggtgtgtggg atctgcatgg aggtggtcta tgagaaagcc aaccccagcg agcgtcgctt 301 tgggatcctc tccaactgca accacaccta ctgtctcaag tgtattcgca agtggagaag 361 tgctaaacaa tttgagagca agatcataaa gtcctgcccc gaatgccgga tcacatctaa 421 ctttgtcatt ccaagtgaga actgggtgga ggagaaagaa gagaagcaga aactcattca 481 gaaatacaag gaggccatga gcaacaaggc gtgcaggtat tttgatgaag gacgtgggag 541 ctgcccattt ggagggaact gtttttacaa gcatgcgtac cctgatggcc gtagagagga 601 gccacagaga cagaaagtgg gaacatcaag cagataccgg gcccaacgaa ggagccactt 661 c //