LOCUS BG078834 604 bp mRNA linear EST 12-MAY-2010 DEFINITION H3032F02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3032F02 5', mRNA sequence. ACCESSION BG078834 VERSION BG078834.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 604) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG078834.1. Other_ESTs: H3032F02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3032 row: F column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..604 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3032F02-5" /db_xref="taxon:10090" /clone="H3032F02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 144 a 148 c 171 g 141 t ORIGIN 1 ggatagtcgg ccacagagcc catggcagtg cccatgatgc tgctcagcct ggtggagcag 61 cttggagaag aagatgagga gctgaccaac atgtatgcag aactagggga ctggtgtgtc 121 cacaggattc ttcagcatgt ccagagggat ggacaagttg tactggagaa tgtatcagag 181 gatggaaaag agcttcctgg ttgccttgga agacatcaga acccaggcca cacactggaa 241 gctggctggt ttctgctcca gtatgccctc aggaaaggtg accccaaact tcgaatgcac 301 atcattgaca agtttctctt attgcctttc cactctggat gggaccctga acatggaggc 361 ctcttctact tccaggatgc ggatggtctc tgccctaccc agctggaatg gaacatgaag 421 ctgtggtggc cacacagtga agccatgatt gccttcctca tgggttatag tgacagtggg 481 gaccctgcct tgctgcatct cttctacaag gtggctgagt acaccttccg ccagtttcgt 541 gatcctgagt atggggaatg gtttggctac ctgaaccaag agggaaaggt ggccctcacc 601 atca //