LOCUS BG078569 600 bp mRNA linear EST 12-MAY-2010 DEFINITION H3029C10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3029C10 5', mRNA sequence. ACCESSION BG078569 VERSION BG078569.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 600) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG078569.1. Other_ESTs: H3029C10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3029 row: C column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..600 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3029C10-5" /db_xref="taxon:10090" /clone="H3029C10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 170 a 145 c 144 g 141 t ORIGIN 1 atactgacca gccagaattt gctactgagc tagcctggga gattgacagg aaagtccttc 61 atcagcaccg gttacagcgc acgccgatca aactccagtg ctttgccttg gacccgcaga 121 cttctgccaa ggaaaccgta ggctacattg ttttggactt aagaacagct caagaaacaa 181 agcaggctcc caaatggtac cagctgctga gtaacaaata caccaagttc aaggccgagg 241 tgcagatcag cctcactttg gagacagata caaaggccca ggtggatagt tataaagcaa 301 aagcagcccc gcctcgggat ggaaaagttc ttgctagcct ggcaggagtt gacccgaagg 361 acattgttgc agtattgaat gaggaaggag gctaccatca gattggacca gcggagcact 421 gtactgaccc ctttattttg tcggtgacga ttgcatttgc cacccagttg gaacagttaa 481 ttccatgtac catgaagctt ccagaaaggc agcctgagtt tttcttttac tattctttac 541 tgggaaacga tgttacaaac gagcctttca gtgatttgat caacccaaac tttgagcccg //